Fig 5

NLGN1 modulates APC localization at the plasma membrane. Confocal microscopy analysis of NLGN1 and APC co-staining in HuTu 80 (A and B), and SNU-C2A cells (C and D). A and C Cells were immunostained with anti APC (magenta) and anti NLGN1 (red) antibodies. Images shown are representative of 1 out of three reproducible experiments. Scale bar: 20 μm. The graphs in B and D show the quantification of APC signal at the plasma membrane using the imageJ Software and values are expressed as mean ± SD (HuTu 80: n = 17; SNU-C2A: n = 15). Mann-Whitney test, two tailed: ** p < 0.01