Fig. 7

CircARID1A-IGF2BP3-SLC7A5 RNA–protein ternary complex regulates the stability of SLC7A5 mRNA in GC. A CircARID1A and SLC7A5 mRNA binding sites predicted using IntaRNA 2.0. B Enrichment of SLC7A5 mRNA by RNA pull-down using circARID1A probes. C RNA-FISH combined with IF to visualize the location of circARID1A, SLC7A5 and IGF2BP3 in SGC7901 and BGC823 cells with or without circARID1A knockdown as indicated. D RIP analysis of SLC7A5 enrichment using GFP pull-downs in SGC7901 cells overexpressing EGFP-tagged IGF2BP3 in SGC7901 cells. E–F RIP analysis of SLC7A5 enrichment using IGF2BP3 pull-downs in SGC7901 cells after (E) IGF2BP3 and (F) circARID1A knockdowns as indicated. G RIP analysis of SLC7A5 enrichment using GFP pull-downs in SGC7901 cells transfected with full-length of IGF2BP3 or truncations as indicated. H–K Stability of SLC7A5 mRNA in SGC7901 and BGC823 cells following (H-I) IGF2BP3 knockdown and actinomycin D treatment and (J-K) following circARID1A knockdown and actinomycin D treatment. The P-values were calculated using the two-tailed Student’s t test. *P < 0.05