Fig. 2

Exogenous DJ-1 enhances the metastasis of ESCC cells in vitro and vivo. A Representative images of transwell assays for bystander ESCC cells co-culture with irradiated cells or exogenous DJ-1. Scale bars, 100 μm. B Representative images of wound-healing assays for bystander ESCC cells co-culture with irradiated cells or exogenous DJ-1. Scale bars, 200 μm. C-D The statistical graphs of transwell assays presented in part A. E–F The statistical graphs of Wound-healing assays presented in part B. G-H Representative IF images and the statistical graph of the 3D tumor spheroid invasion assay for bystander cells (green) co-culture with irradiated cells or exogenous DJ-1. Scale bars, 200 μm. I-J Representative photographs of metastatic nodules were taken from nude mice injected DJ-1 protein by an IVIS imaging system. Quantification of the luciferase was shown. K H&E staining was used to characterize the lung metastatic nodules. Scale bar, 2 mm. **p < 0.01, ***p < 0.001