Fig. 4

Exogenous DJ-1 promotes metastasis by binding to HSC70 in ESCC cells. A Results of proteins identified from LC–MS/MS ordered by the score of iBAQ. B The targets of intersection between proteins identified from LC–MS/MS and DEGs screened from RNAseq. C Representative IF images of colocalization of DJ-1 (red) and HSC70 (green) in K150 and E109 cells detected using immunofluorescence microscopy. Scale bar, 20 μm. D Coimmunoprecipitation analysis demonstrated that exogenous DJ-1 could interact with HSC70. E–H Representative images and the statistical graph of transwell assays for bystander K150 and E109 cells transfected with siRNA targeting HSC70. Scale bars, 100 μm. ***p < 0.001