Fig. 5

Exogenous DJ-1 acting on HSC70 promotes Smad3 phosphorylation and nuclear aggregation in ESCC cells. A Western blot results of Smad3 phosphorylation status relative to total Smad3 in K150 and E109 cells transfected with siRNA targeting HSC70 at the timing (0, 15, 30, 60, 120 and 240 min) after DJ-1 stimulation. B Representative IF images of p-Smad3 (green) and HSC70 (red) expression in K150 and E109 cells transfected with siRNA targeting HSC70 at the timing (0, 15 and 30 min) after DJ-1 stimulation. Scale bar, 20 μm. C Coimmunoprecipitation analysis demonstrated that HSC70 could interact with Smad3 in attendance of DJ-1. D-E Representative images and the statistical graph of transwell assays for bystander K150 and E109 cells transfected with siRNA targeting Smad3. Scale bars, 100 μm. **p < 0.01, ***p < 0.001, ****p < 0.0001