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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Irradiation induces DJ-1 secretion from esophageal squamous cell carcinoma cells to accelerate metastasis of bystander cells via a TGF-β1 positive feedback loop

Fig. 8

Blocking TSP1 reduces phosphorylation and nuclear aggregation of Smad3 and metastasis in vivo. A Western blot results of prominent proteins correlated with the TGF-β1 signaling pathway in bystander K150 cells transfected with siRNA targeting THBS1 at the timing (0, 15, 30, 60, 120 and 240 min) after DJ-1 stimulation. B-C Representative IF images of p-Smad3 (green) expression in K150 cells transfected with siRNA targeting THBS1 at the timing (240 min) after DJ-1 stimulation. Scale bar, 20 μm. D Western blot results of prominent proteins correlated with the TGF-β1 signaling pathway in bystander K150 cells measured by LSKL at the timing (0, 15, 30, 60, 120 and 240 min) after DJ-1 stimulation. E–F Representative IF images of p-Smad3 (green) expression in K150 cells measured by LSKL at the timing (240 min) after DJ-1 stimulation. Scale bar, 20 μm. G-H Representative photographs of metastatic nodules were taken from nude mice injected LY2109761 or LSKL by an IVIS imaging system. Quantification of the luciferase was shown. I H&E staining was used to characterize the lung metastatic nodules. Scale bar, 2 mm. ***p < 0.001, ****p < 0.0001

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