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Table 1 Primary and secondary antibodies used in this study

From: APE1 redox function is required for activation of Yes-associated protein 1 under reflux conditions in Barrett’s-associated esophageal adenocarcinomas

Antibody

Source

Catalog#

Species

Dilution

Applicationa

YAP

Cell signaling

14074

Rabbit

1:1000

1:200

WB

IHC

YAP

Santa Cruz

sc-101199

Mouse

1:100

IF

APE1

Invitrogen

MA1-440

Mouse

1:7000

WB

APE1

Invitrogen

PA5-29157

Rabbit

1:200

IF

β-actin

Sigma Aldrich

A5441

Mouse

1:10,000

WB

P84

Genetex

GTX70220

Mouse

1:1000

WB

β-tubulin

Cell signaling

2128

Rabbit

1:1000

WB

β-TrCP

Invitrogen

37–3400

Mouse

1:100

PLA

β-TrCP

Cell signaling

4394

Rabbit

1:1000

WB

Flag antibody

Sigma Aldrich

F1804

Mouse

2 µg

IP

Ubiquitin

Cell signaling

43124

Rabbit

1:1000

WB

Anti-rabbit IgG HRP-linked Antibody

Cell signaling

7074

Goat

1:5000

WB

Anti-mouse IgG HRP-linked Antibody

Cell signaling

7076

Goat

1:5000

WB

Alexa Fluor 488 goat anti-mouse IgG

Invitrogen

A-11029

Goat

1:200

IF

Alexa Fluor 568 goat anti-rabbit IgG

Invitrogen

A-11011

Goat

1:200

IF

Mouse Anti-Rabbit IgG (Light-Chain Specific) HRP Conjugate

Cell signaling

93702

Mouse

1:1000

WB

Goat Anti-Mouse IgG (Light-Chain Specific) HRP Conjugate

Cell signaling

91196

Goat

1:1000

WB

  1. aIF Immunofluorescence, WB Western blot, IP Immunoprecipitation, IHC Immunohistochemistry, PLA Proximity ligation assay