Fig. 6

T cell activation bioassays in the presence of 5T4-expressing target cells. A Schematic representation of TCR/CD3 activation in the presence of 5T4-expressing cells by T cell activation bioassay (NFAT). B Genetically engineered Jurkat T cells which expresses a luciferase reporter driven by a NFAT-response element (NFAT-RE) (“TCR/CD3 effector cells”) were incubated with increasing concentrations of the indicated tribodies in the presence of CHO-K1-5T4 cells. After 4 h incubation at 37℃, Bio-Glo™ reagent was added and luminescence was measured by a luminometer. Luminecence values were reported as the mean of determinations obtained in three independent experiments. C Table reporting the EC50 (nM) values for 5T4-mediated TCR/CD3 activation of each indicated construct