Fig. 2

ETV1 promotes HCC metastasis in vitro and in vivo. (A) The efficiency of ETV1-overexpression and ETV1-knockdown in the indicated HCC cells was detected by western blotting. (B) Effects of ETV1 overexpression and knockdown on HCC cell migration and invasion. Scale bar, 100 μm. (C-H) In vivo metastatic assay. (C) The representative BLI images in the liver were shown nine weeks after implanting with indicated cells. (D) The bioluminescent signals were used to show the growth rate of liver tumors. (E) The number of metastatic lesions in the lung tissues. (F) The occurrence of lung metastasis. (G) The OS of different groups in the in vivo models. (H) Representative images of H&E staining of lung samples (indicated by arrowheads) from each group. Scale bars, 1mm(upper), 100μm (lower). * Represented p < 0.05. All data were displayed as Mean ± SD