Fig. 3
ETV1 transcriptionally upregulates PTK2 and c-MET expression. (A) Human Liver Cancer PCR Array was employed for detecting the upregulated genes in PLC/PRF/5-ETV1 and down-regulated genes in MHCC97H-shETV1 cells compared with controls (fold change ≥ 2.0). Overlapping genes were shown in the Venn diagram. (B-C) Compared with the controls, PTK2 and c-MET were upregulated when ETV1 was overexpressed in PLC/PRF/5 cells, and they were down-regulated when ETV1 was knocked down in MHCC97H cells, detected by qRT-PCR and western blotting. (D) Relative luciferase activity of PTK2 and MET promoter reporter vectors after cotransfection of pCMV-ETV1 and PTK2 or MET plasmid constructs in PLC/PRF/5 cells. (E-F) Relative luciferase activity of indicated PLC/PFR/5 cells cotransfected with pCMV-ETV1 and truncated or mutated luciferase constructs. (G-H) ChIP assay of ETV1 binding to the PTK2 or MET promoter in PLC/PRF/5 cells, as well as HCC specimens. * Represented p < 0.05. All data were displayed as Mean ± SD