Fig. 7

Inhibition of macrophage activation after co-culture with colon cancer cells. a, Quantitation data based on the mean levels of western blots at 48 hs after transfaction (n = 3). b, Cell morphology of macrophages. THP-1 cells were grown and treated with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA) for 48 h. c, qRT-PCR. The co-cultured THP-1 cells were analyzed for mRNA levels of the M1 or M2 macrophages markers using qRT-PCR. d, Western blot. The co-cultured THP-1 cells were analyzed for CD163 and iNOS proteins using Western blot. e, Flow cytometry. The co-cultured THP-1 cells were analyzed for CD86⁺ macrophages. NC, negative control cells transfected with negative control siRNA; KD, knockdown of PCSK9 cells transfected with PCSK9 siRNA. *p < 0.05; **p < 0.01; ***p < 0.001