Fig. 5

NRF1-DDR4 regulatory axis in HCC. A Venn plot showing the overlap of LINC01132 binding proteins and transcription factors, cofactors binding to DPP4 promoter region. B SDS-PAGE staining results of LINC01132 pull down assay. C Immunoblotting for the specific associations of NRF1 or KDM5B with biotinylated-LINC01132 from streptavidin RNA pull-down assays. D-E RIP assays were performed using the indicated antibodies. Real-time PCR was used to detect LINC01132 enrichment, using IgG antibody as the control. D for NRF1 and E for KDM5B. F Integrative Genomics Viewer of NRF1 binding around the TSS of DPP4 in HCC cell lines. G Immunoblotting of NRF1 and KDM5B in samples from DPP4 RIP assays with LINC01132 overexpression and knock down