Fig. 5
MET-CAR-T killing activity on human cancer cells overexpressing the MET receptor. (A) MET-CAR-T specific killing activity at different Effector:Target ratio versus NCI-H226 and NCI-H441 human lung carcinoma cells, and Caki-1 human renal carcinoma cells. (B) Analysis of cell viability after 3 days of treatments with a MET-specific small molecule TKI (JNJ-605) or with a MET-blocking antibody (MvDN30) on NCI-H226, NCI-H441, and Caki-1 cells. Graph reports values as percentage of the untreated controls (CTRL). MET 'addicted' EBC-1 cells has been included in the assay as positive control. (C) MET-CAR-T specific killing activity at different Effector:Target ratio versus primary gastric carcinoma cells expressing different levels of MET receptor at the cell surface. GTR-164: MET negative cells; GTR-468 and GTR-210 MET overexpressing cells. (D) Analysis of cell viability after 3 days of treatments with different concentrations of a MET targeting small molecule TKI (Crizotinib). Graph reports values as percentage of the untreated controls. NTD: Not-transduced T cells, #948/#949: MET-CAR-T. Bars: Standard Deviations. Statistical significance was calculated by Two-way Anova, corrected Bonferroni. Stars indicated P values: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001