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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Combined alteration of lamin and nuclear morphology influences the localization of the tumor-associated factor AKTIP

Fig. 1

3D SIM imaging of the localization of AKTIP in HeLa cells (AB) 3D rendering (A) and projections of an extended section viewed from orthogonal planes and magnified sections (B) from super resolution images of a HeLa nucleus labelled with anti-lamin B1 and anti-AKTIP antibodies. C Quantification of the percentage of AKTIP foci at a distance below or above 0.5 µm from the lamin B1 signal. D Projection of an extended section of a nucleus labelled with anti-lamin A/C and anti-AKTIP antibodies and magnified sections. E Projections of an extended section viewed from orthogonal planes of a nucleus labelled with anti-TPR and anti-AKTIP antibodies and magnified sections (F) Co-localization between AKTIP and the indicated proteins from (D) and (E) quantified as Pearson correlation coefficient. Each dot corresponds to a nucleus. Mean ± SEM are shown, images from 9 nuclei per cell condition were analyzed. ***p < 0.001 in unpaired Student t-test

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