Fig. 3
From: Wild-type IDH1 inhibition enhances chemotherapy response in melanoma
IDH1 supports mitochondrial function under stress. A Principal-component analysis (PCA) of metabolites analyzed by LC-MS/MS performed on A375 cells, after transfection with si.IDH1 and si.CTRL (n = 3 samples). B A heatmap of the top 50 metabolites with the greatest change in A375 cells after transfection with si.IDH1 versus si.CTRL (n = 3 independent samples) under 2.5 mM glucose and analyzed by LC/MS. The scale is log 2 fold-change. C Relative levels of TCA cycle metabolites from A375 after transfection with si.IDH1 and si.CTRL under 2.5 mM glucose for 12 hours. D Metabolite set enrichment analysis of A375 cells. E Representative oxygen consumption rate (OCR) in A375 cells transfected with si.IDH1 and si.CTRL, and cultured in 2.5 mM glucose for 24 hours. Treatment with mitochondrial inhibitors are indicated: oligomycin (Oligo), FCCP, antimycin A and rotenone (Anti/Rot) and F Basal mitochondrial OCR. Each data point represents the mean ± SEM of three independent experiments. N.S., nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001