Fig. 1
FAM83H-AS1 is upregulated in PDAC tissues and positively correlated with poorer prognosis. A Venn diagram of abnormally expressed lncRNAs in three comparison models (PDAC vs. pancreas, T2N0M0 vs. T2N1M0/T2N0M1/T2N1M1 and T3N0M0 vs. T3N1M0/T3N0M1/T3N1M1) based on RNA-seq data from the TCGA database. B, C FAM83H-AS1 expression in the combined TCGA and GTEx cohort (B) and the cohort from Tongji Hospital (C). D Serum FAM83H-AS1 expression was tested in patients with PDAC and benign nonpancreatic disease from Tongji Hospital. E RT–qPCR results showing the expression of FAM83H-AS1 in HPDE and PDAC cell lines (Panc 03.27, MIA PaCa-2, PANC-1, SW 1990, AsPC-1, BxPC-3 and Capan-2). F-G A survival analysis was conducted to illustrate the relationship between the level of FAM83H-AS1 and the overall survival of patients in the TCGA cohort (F) and the cohort from Tongji Hospital (G). H Nuclear/cytosol fractionation followed by RT-qPCR analysis of the level of FAM83H-AS1 in the nucleus and cytoplasm. I Combination of FISH and IF assays showing the subcellular distribution of FAM83H-AS1; scale bar: 50 μm. *P < 0.05 and ***P < 0.001