Fig. 2

FAM83H-AS1 promotes PDAC cell proliferation in vitro and tumor growth in vivo. A The overexpression and knockdown of FAM83H-AS1 in stably transfected PANC-1 and SW 1990 cells was confirmed by RT-qPCR analysis. B A combination of EdU and IF assays were used to compare the proliferative activity between the sh-FAM83H-AS1#1/#2 groups and the sh-Control group in SW 1990 cells; scale bar: 50 μm. C Colony formation assays showing the colony numbers of tumor cells and analysis of the differences between groups. D-G A xenograft tumor model was used to analyze the effects of FAM83H-AS1 interference on tumor growth by monitoring the tumor volumes and weight and analyzing the expression of Ki-67 and PCNA in tumor tissue by IHC; scale bar: 100 μm. *P < 0.05, **P < 0.01 and ***P < 0.001