Fig. 3

FAM83H-AS1 promotes PDAC cell invasion and metastasis in vitro and in vivo. A Transwell assays were conducted to determine the invasion and migration of FAM83H-AS1 shRNA-transfected PDAC cells; scale bar: 100 μm. B Wound healing assays were conducted to evaluate the migration of FAM83H-AS1 shRNA-transfected PDAC cells; scale bar: 100 μm. C Western blotting was performed to assess the expression of E-cadherin, N-cadherin and Vimentin in PDAC cells after FAM83H-AS1 knockdown. D, E The mouse model was established by tail vein injection of SW 1990 cells with FAM83H-AS1 knockdown. Representative bioluminescence images (D), representative images of the lungs (E, upper panel) and H&E staining of lung tissues (E, lower panel) are shown. F The average weight curve was constructed to assess the effect of the metastasis of PDAC cells with FAM83H-AS1 knockdown on the nutritional condition and growth of mice. G Survival analysis was performed on mice bearing the indicated PDAC cells immediately after injection. *P < 0.05, **P < 0.01 and ***P < 0.001