Fig. 5From: LncRNA FAM83H-AS1 promotes the malignant progression of pancreatic ductal adenocarcinoma by stabilizing FAM83H mRNA to protect β-catenin from degradationFAM83H is upregulated in PDAC tissues and cells and promotes PDAC progression. A, B Expression of FAM83H in the combined TCGA and GTEx cohort (A) and the cohort from Tongji Hospital (B). C Western blot results showing that FAM83H was expressed at higher levels in PDAC tissues than in adjacent tissues. D, E Kaplan–Meier analysis and long-rank test determining the prognostic significance of FAM83H in PDAC patients from the TCGA cohort (D) and Tongji Hospital cohort (E), respectively. F RT–qPCR and Western blot results showing FAM83H expression in HPDE and PDAC cell lines (Panc 03.27, MIA PaCa-2, PANC-1, SW 1990, AsPC-1, BxPC-3 and Capan-2). G CCK-8 assays were performed to compare the cell viability between the LV-FAM83H group and the LV-Control group and between the sh-FAM83H#1/#2 group and the sh-Control group. H Transwell assays were used to determine the invasion and migration of FAM83H shRNA-transfected PDAC cells. scale bar: 100 μm; I Western blots showing the expression of β-catenin, E-cadherin, N-cadherin and Vimentin in PDAC cells with FAM83H knockdown. *P < 0.05, **P < 0.01 and ***P < 0.001Back to article page