Fig. 4

AADAC reduces lipid peroxidation. A Significantly enriched lipid species detected by untargeted lipidomic analysis in sh-AADAC HCT116 cells compared to shNC cells. B Heatmap of PUFA-containing PEs significantly enriched in sh-AADAC HCT116 cells. C Representative ROS levels in shNC and sh-AADAC HCT116 cells. D Representative images and quantification of lipid droplets in control, sh-AADAC, and AADAC-oe (ADOE) HCT116 cells (scale, 100 μm). E Venn diagram of significantly enriched and proferroptotic lipid species among 84 upregulated lipids in sh-AADAC cells. F Fold change, p value, and VIP value of 5 proferroptotic lipid species in (E). Fold change > 1.5; FDR-related p value < 0.05; VIP value of lipidomic analysis > 1. G Relative cellular MDA levels in shNC and sh-AADAC HCT116 cells treated with 15 μM erastin. H Relative levels of GSH, GSSG, and the GSH/GSSG ratio in sh-AADAC HCT116 cells. Data are shown as the mean ± SD. Significance was calculated by a two-tailed ratio t test (right of C, left of D, G-H). p value < 0.001 (***), p value < 0.01 (**), p value < 0,05 (*), ns (not significant)