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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: AADAC protects colorectal cancer liver colonization from ferroptosis through SLC7A11-dependent inhibition of lipid peroxidation

Fig. 5

AADAC upregulates SLC7A11 to suppress ferroptosis. A Cell viability of sh-AADAC HCT116 and ADOE SW480 cells treated with graded concentrations of erastin. B Protein expression of ferroptosis-related genes in sh-AADAC HCT116 cells and ADOE SW480 cells. C Protein expression of SLC7A11 in control, ADOE, and ADOE + sh-SLC7A11 (ADSL) SW480 cells. D Relative levels of GSH, GSSG, and the GSH/GSSG ratio in control, ADOE, and ADSL SW480 cells. E Relative MDA levels in control, ADOE, and ADSL SW480 cells treated with 15 μM erastin. F-G Representative images, HE staining (scale bar, 1 mm) (F) and quantification (G) of liver colonies from CRC liver colonization mice model with different SW480 cells (control, ADOE, and ADSL). H Representative IHC staining of AADAC and SLC7A11 expression in the LMs of CRLM patients (left scale bar, 500 μm; right scale bar, 100 μm). I Pearson correlation between IHC H scores of AADAC and SLC7A11 expression in LMs. Data are shown as the mean ± SD. Significance was calculated by two-way ANOVA and two-tailed ratio t test (A, D, E, G). p value < 0.001 (***), p value < 0.01 (**), p value < 0,05 (*), ns (not significant)

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