Fig. 1

Identification of circPAK1. A Flow diagram of circRNAs screening in 3 pairs HCC tissue. B Illustration of the annotated genomic region of circPAK1 Sanger sequencing was conducted to confirm the ‘head-to-tail’ splicing of circPAK1. C The divergent primers detected circPAK1 in cDNA but not in gDNA, GAPDH was used as a negative control. D Relative RNA level of circPAK1 and linear PAK1 in different time point. E and F showed the relative RNA level of circPAK1, linear PAK1 and GAPDH treated with RNase R. G The nuclear mass separation assay and H FISH showed that the sub-cellular distribution of circPAK1 was mostly present in the cytoplasm. *p < 0.05; **p < 0.01; ***p < 0.001. Data were shown as mean ± SEM