Fig. 5

CircPAK1 enhances HCC tumor growth and metastasis in vivo and CS/si-circPAK1 nanocomplexes inhibits HCC growth and metastasis in vivo. A Nude mice were subcutaneously injected with circPAK1 stable knockdown or overexpression cells. The tumor volume and average weight were determined. B IHC analysis of Ki-67 in the tumors derived from mice, scale bar, 50 μm. C CircPAK1 stable knockdown or overexpression cells were injected into the tail vein of nude mice to induce lung metastasis, and gross lung tissues were obtained; liver metastatic nodules were subjected to HE staining as indicated, scale bar, 200 μm. D The loading efficiency of CS. E TEM images of CS/si-circPAK1 nanocomplexes (CS/ si-circPAK1 = 50/1). F The flow diagram showed the scheme of intratumorally/intravenously with saline, si-circPAK1 or CS/si-circPAK1 into mice. G and H Weight volume and weight change after 20 days of treatment with saline, si-circPAK1 or CS/si-circPAK1 for xenograft tumors or lung metastasis models. The tumor volume and average weight were determined; gross lung tissues were obtained and liver metastatic nodules were subjected to HE staining as indicated, scale bar, 200 μm. *p < 0.05; **p < 0.01; ***p < 0.001. Data were shown as mean ± SEM