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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: CircPAK1 promotes the progression of hepatocellular carcinoma via modulation of YAP nucleus localization by interacting with 14-3-3ζ

Fig. 7

CircPAK1 competitively binds 14–3-3ζ with YAP thus reduces the phosphorylation of YAP by p-LATs. A RIP-qPCR was performed to explore the association between circPAK1 and YAP. B Silver-stained SDS-PAGE gel-containing proteins derived from RNA pulldown by circPAK1 probe and negative control. The red rectangle was used for mass spectrometric analysis. C Western blot analysis showed the specific association of circPAK1 with 14–3-3ζ. D qRT-PCR analysis of the RNAs derived from the RIP assays. E A proposed model for 14–3-3ζ mediation of p-LATS-YAP interactions. F Western blot analysis identified 14–3-3ζ protein expression level in LM3 transfected sh-circPAK1. G CircPAK1 stable knockdown LM3 cells were subjected to IP using 14–3-3ζ antibody or control IgG, followed by IB with 14–3-3ζ, p-LATS1/2 and YAP antibody, to confirm and determine the 14–3-3ζ- p-LATS-YAP protein complex. H CircPAK1 stable knockdown LM3 cells were subjected to IP using YAP antibody or control IgG, followed by IB with 14–3-3ζ, p-LATS1/2 and YAP antibody. *p < 0.05; **p < 0.01; ***p < 0.001. Data were shown as mean ± SEM

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