Fig. 6

GSDMB expression promotes Rab7 activation in response to anti-HER2 drugs. A Co-immunoprecipitation assay of Rab7-GTP levels in shNTC and shGB2 HCC1954 cells after the treatment with lapatinib (2 µM) and the combined treatment. Statistical significance was determined by two-tailed unpaired t-test (**P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. B GSDMB, p62 and LC3B protein levels in HCC1954 cells treated for 72 h with or without chloroquine (10 µM). C-D Representative multiplex immunofluorescence images of GSDMB (green), Rab7 (red) and LC3B (deep red) co-expression in adjuvant breast (C) and gastric carcinomas (D). Scale bar, 20 µm. Nuclei were counterstained with DAPI. Representative pie charts of Rab7 and GSDMB co-expression statistics and its relationship with relapse in adjuvant breast (C) and gastric carcinomas (D) (see Supplementary Tables 1, 2 and 5 for extra clinical features and statistics). CQ, chloroquine. Lap, lapatinib