Fig. 8

In situ characterization of CD4⁺ and CD8⁺ T-cell migration by confocal microscopy. a Representative spleen images. Tissues were processed for histologic analysis and triple stained with hoechst33342 (blue), hCD4 (green), and hCD8 (red). Scale bar = 0.5 mm; b Magnification of spleen area treated by hPBMCs + DC-iMBs. Scale bar = 40 μm; c Splenic quantification of fluorescence intensity ratios (hCD4/Hoechst and hCD8/hoechst33342) for all animal groups; d Representative tumor sections with respective magnified areas. Scale bars = 0.5 mm and 40 μm, respectively. e Quantification of fluorescence intensity ratios (hCD4/Hoechst and hCD8/hoechst33342) in tumor sections for all animal groups. **p < 0.005