Fig. 6
The combination of lycorine hydrochloride and HA14–1 enhances the therapeutic effect on gastric cancer. a Apoptosis of MKN-45 and SGC-7901 cells treated with LH (20 μM), HA14–1(9 μM) or LH (20 μM) + HA14–1(9 μM) for 48 h were examined by trypan blue staining. DMSO was used as control. Apoptotic rate of MKN-45 and SGC-7901 cells was quantified. b Western blotting was used to detect the expression of apoptotic protein, including BCL2, MCL1, C-Caspase 9, C-Caspase 3 and C-PARP in MKN-45 and SGC-7901 cells after 48 h of treatment with LH (20 μM), HA14–1 (9 μM) or LH (20 μM) + HA14–1 (9 μM). DMSO was used as control. c Tumor volume and weight of indicated mice. DMSO was used as control. The efficiency index (q) analysis of LH (30 mg/kg) combined with HA14–1 (2.5 mg/kg) treatment in the weight of PDX tumors through Jin’s formula. d The weight of the mice treated with DMSO, HA14–1, LH or LH + HA14–1 was measured. e IHC of MCL1 and Ki67 in indicated tumors. Scale bar =20 μm. Gray value of IHC positive signal in panel was quantified. All data were analyzed by unpaired Student’s t-tests and were shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001