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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: FOXD1-dependent RalA-ANXA2-Src complex promotes CTC formation in breast cancer

Fig. 2

FOXD1 promotes the CTC formation in BC via regulating EMT. A Bar graphs showing the CTC number from mice bearing a MDA231 control or FOXD1 knockdown primary tumor at week 9. The CTCs were captured by the microfluidic CTC chip. Error bars, SD. n = 5. ***p < 0.001 by Student’s t-test. B Images of bioluminescence imaging (BLI) of the lung from two indicated groups at week 9. Bar graph showing normalized lung photons detected by BLI. Error bars, SEM. n = 5. **p<0.01 by Mann-Whitney test. C Representative images of BLI of mice on 1, 3, 5, 7, 9 weeks after orthotopic injection of indicated cells. Bar graphs showing normalized tumor photons from two indicated groups. Error bars, SEM. n = 5. *p<0.05 by Student’s t-test. D Representative images of the xenograft tumors isolated from two indicated groups at week 9. The tumor volumes of xenograft tumors isolated from shCtrL and shFOXD1 groups were analyzed by bar graph. Error bars, SEM. n = 5. *p<0.05 by Student’s t-test. E Representative images of zebrafish from two indicated groups. The numbers of control or FOXD1 knockdown cells (white arrows indicated) intravasated into circulation were showed by bar graph. Scale bar, 200 μm. Error bars, SD. n = 3. **p < 0.01 by Student’s t-test. F Cell migration capacity of FOXD1 knockdown cells (MDA231 and MDA468) or FOXD1 overexpression cells (MCF7) and control cells was determined by the transwell. Representative photographs of migratory cells are shown. Scale bar, 250 μm. Error bars, SD. n = 3. ***p < 0.001 by Student’s t-test. G Expression of EMT relative proteins (E-cadherin, Vimentin, N-cadherin, β-catenin, Snail) in CtrL and FOXD1 knockdown cells (MDA231 and MDA468), Ctrl and FOXD1 overexpression cells (MCF-7) were analyzed by Western blot. H IHC analyzed E-cadherin and Vimentin expression in primary tumors with CTC ≥ 5 or CTC<5. Scale bar, 50 μm. Error bars, SD. n = 5. *p<0.05, ***p < 0.001 by Student’s t-test

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