Fig. 4

BET inhibitor works synergistically with proteasome inhibitor on suppressing G3-MB. a Box plots showing PSMB5 mRNA levels of four MB subgroups or NC in the indicated MB datasets. b Kaplan-Meier analysis of the overall survival of MB patients stratified by PSMB5 mRNA levels in Cavalli dataset. c Cell viability (top) and CI (bottom) of MB002 and D283 cells treated with JQ1 and Marizomib at the indicated concentrations for 72 h. d FACS analyses of cell proliferation (top) and apoptosis (bottom) of MB002 cells treated with Marizomib and JQ1 at the indicated concentrations. e RT-qPCR analysis of the selected ER stress genes in MB002 and D283 cells treated with JQ1 (1 μM for MB002, 2 μM for D283) and Marizomib (4 nM for MB002, 50 nM for D283) individually or in combination for 8 h. f Kaplan-Meier survival curve of nude mice carrying orthotopic xenografts of MB002-GFP-luc cells treated with JQ1 and Marizomib individually or in combination as indicated. g-h The tumor growth of xenografted nude mice treated as described in (f) was monitored by IVIS weekly. The collected mice images with corresponding signal scale bars measured in p/s are shown in g. Crossed white box indicates death of the treated mouse. Box plots showing the signals of total bioluminescence flux intensity for xenografted nude mice of each treatment condition and the data are presented as mean ± SEM in h. All RT-qPCR and cell viability assays were performed in triplicate and the data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA (a and e), two-sided log-rank test (f) and two-way ANOVA (h), respectively