Fig. 4

DZD2269 enhances the efficacy of radiotherapy (RT) in syngeneic mouse in vivo models. (A-B) RM-1 tumor cells were irradiated with 20 Gy radiation and were co-cultured anti-CD3/CD28 antibodies activated mouse splenocytes. (A) IFN-γ in supernatant was measured 72 h after co-culture. (B) IL-2 in supernatant was measured 24 h after co-culture. Significance Statistically significant difference was calculated by using one way ANOVA (*: p < 0.05, ***: p < 0.001, ****: p < 0.0001, ns: no significance). (C) Pancreatic Pan02 and (D) Prostate RM-1 models were established in C57BL/6 mice. Pan02 tumor-bearing mice received daily fixed 5 Gy radiation at day 1, day 2 and day 3 and RM-1 tumor-bearing mice received 8 Gy radiation at day 1. DZD2269 at the indicated dosage was given from day 1 to the end of the experiment. Error bars represent standard error of the mean. Statistically significant difference was calculated by using two-way ANOVA (*: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001). bid: twice daily. (F) Tumor samples from Pan02 model were harvested at the end of the experiment. Infiltrated CD8 + and CD4 + T cells were determined by IHC. N = 4 mice per group. One way ANOVA was conducted to compare the combination group versus vehicle or single-agent groups (*: p < 0.05, **: p < 0.01)