Fig. 2

miR-130b-3p positively modulates the pro-angiogenic ability of mTORC1-activated cells. A-C Tsc2 − / − MEFs were transduced with lentivirus expressing an antisense sequence against miR-130b-3p (anti-miR-130b) or a scrambled sequence (control). D-G Tsc2 + / + MEFs were infected either with a lentivirus encoding miR-130b-3p (miR-130b) or a scrambled miRNA sequence (control). A and D The expression of miR-130b-3p was determined by qRT-PCR. B, C, E, and F The effect on angiogenesis was tested by tube formation assay (B and E, scale bar, 50 μm) and CAM assay (C and F). Representative images (left panels) and quantifications (right panels). G mRNA levels of the indicated angiogenesis-related genes were measured by qRT-PCR. H–L Tumor growth of mice subcutaneously injected with NTC/T2 null cells stably transduced with anti-miR-130b-3p or control lentiviruses. N = 5 for each group. (H) Tumor pictures. Scale bar, 1 cm. I Tumor growth curves. J Tumor weight. K Representative HE and IHC staining for CD31 of the indicated tumor tissues. Scale bar, 50 μm. L miR-130-3p levels in the indicated tumor tissues were determined by qRT-PCR. Data indicate mean ± SD of 3–5 replicates. ** P < 0.01; *** P < 0.001; **** P < 0.0001