Fig. 3

Breast cancer cell migration relies on a functional oxidative phosphorylation (OXPHOS) system. (A) Hypoxia generation by a cluster of T6 cells placed under confinement as visualised using the Visisens device (see Methods). Left panel: phase contrast image at 0 min. Right panel: detection of hypoxia generated at 0, 5 and 10 min after confinement. Scale Bar (0.5 mm). (B) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with antimycin A (AA, 1 µM) or oligomycin D (OD, 0.5 µM). Scale bars 0.5 mm. See Sup. Figure 3 for the other tumour cells. (C) Cell distribution from the centre of the well at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 1 µM AA (yellow) or 0.5 µM OD (blue). Y-axis scale is expressed in arbitrary unit