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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: EGFR-dependent aerotaxis is a common trait of breast tumour cells

Fig. 5

Aerotaxis of breast cancer cells is dependent on EGFR activation. (A) Immunofluorescence imaging of EGFR in MCF10A cells in the absence of EGF (-EGF), upon activation by EGF (+ EGF), or EGF + cetuximab, a monoclonal EGFR neutralising antibody (+ EGF + Cetux), (B) Immunofluorescence imaging of EGFR in the indicated tumour cells in their culture medium supplemented with EGF. Scale bars, 25 μm and 10 μm for high magnification views inserted in the upper-right corner of each image (x2.5). Steady-state EGFR (-EGF and + EGF/+Cetux conditions) is located at the plasma membrane, whereas it appears as intracellular dots upon activation (+ EGF condition). (C) Aerotactic migration at 48 h of the indicated tumour cells either untreated (Mock), treated with 25 µg/mL or with 12.5 µg/mL cetuximab (Cetux). Scale bars 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) at 48 h in the Mock condition (carmine, mean of three independent experiments), in cells treated with 25 µg/mL (blue) or with 12.5 µg/mL (yellow). Y-axis scale is expressed in arbitrary unit. See Figure S5 for the other tumour cells

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