Fig. 8
From: EGFR-dependent aerotaxis is a common trait of breast tumour cells
Aerotactic migration of Hs578T and MDA-MB-231 is independent of EGFR activation. (A) Representative images taken at 48 h of MCF10A, Hs578T and MDA-MB-231 cell lines subjected to the aerotactic assay incubated in MCF10A or BC medium supplemented or not with cetuximab as indicated. Contrary to MCF10A, aerotactic migration of both HS578T and MDA-MB-231 is not affected by cetuximab. Scale bars, 0.5 mm. (B) Cell distribution from the centre of the cell cluster (in mm) at 0 h (green) and 48 h (carmine) in the -cetuximab condition and at 0 h (yellow) and 48 h (blue) in the + cetuximab condition is indicated as graphs (mean of three experiments). Y-axis scale is expressed in arbitrary unit. To assess the significant difference between the plus and minus cetuximab conditions at 48 h, a Student t-test was performed on D5% values. The p-values are indicated within graphs. NS non-significant