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Table 2 Summary of the aerotactic potential of breast cancer cell lines

From: EGFR-dependent aerotaxis is a common trait of breast tumour cells

  

Aerotaxis

 

Profile

Cell lines

Medium - EGF

Medium + EGF

Medium (± EGF) + cetuximab

total EGFR

1

MCF10A

-

+++

p < 0.001

(+ EGF): -

(p < 0.001)

++

SK-BR3

-

++

p < 0.001

NI

+

BT-549

-

+

p < 0.001

NI

+++

BT-474

-

+

p < 0.001

NI

±

BT-20

-

±

p < 0.001

NI

+++

CAL-51

-

±

p < 0.05

NI

++

2

CAMA1

-

-

NS

NI

-

MCF7

-

-

NS

NI

-

MDA-MB-453

-

-

NS

NI

-

MDA-MB-361

-

-

NS

NI

±

3

MDA-MB-231

+++

+++

NS

(- EGF): +++

NS

++

MDA-MB-157

+

+

NS

ND

++

T47D

+

+

NS

ND

++

4

Hs578T

+++

++++

p < 0.001

(- EGF): +++

NS

+++

MDA-MB-468

+

++

p < 0.001

ND

++++

  1. -, ±, +: Existence or not, and intensity of the aerotaxis
  2. Column “Aerotaxis + EGF”: intensity of aerotaxis with EGF and statistical difference with condition -EGF.
  3. Column “Aerotaxis + cetuximab”: intensity of aerotaxis in the presence of cetuximab in the medium ± EGF and statistical difference with the corresponding medium ± EGF.
  4. p: result of the Student t-test on D5% values.
  5. NS: non-significant.
  6. ND: not determined (usually when migration was not fast enough to expect a significant difference).
  7. NI: no interest when there is no migration to inhibit.
  8. Column total EGFR: Intensity of EGFR expression observed by Western blot. For full results, see Figs. 7 and 8.