Fig. 2
From: CircLRFN5 inhibits the progression of glioblastoma via PRRX2/GCH1 mediated ferroptosis
CircLRFN5 inhibits GSCs viability, proliferation, neurospheres formation and stemness. a qPCR showed the expression of circLRFN5 in six GBM patients derived GSCs. b, c MTS assays showed the cell viabilities of GSC51 (b) and GSC58 (c) after circLRFN5 overexpression. d Representive EdU assays showed the proliferation of GSC51 and GSC58 after circLRFN5 overexpression. Scale bar = 50 μm. e Representive neurospheres formation assays showed the relative sizes of neurospheres after circLRFN5 overexpression in GSC51 and GSC58. Scale bar = 20 μm. f ELDA assays showed the neurospheres formation abilities of GSC51 (left) and GSC58 (right) after circLRFN5 overexpression. g Western blotting detects the stemness markers of GSCs (NANOG, OCT4, CD133 and SOX2) after circLRFN5 overexpression in GSC51 and GSC58. h Cell cycle assays showed the cell cycle distributions of GSC51 and GSC58 after circLRFN5 overexpression. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001