Fig. 8

CircLRFN5 regulates tumorigenesis of GSCs in vivo. a, c Representative photographs showed the sizes of intracranial tumors in the coronal position of the vector control group, circLRFN5 overexpressed group and combined with the PRRX2 or GCH1 overexpression group. Scale bar = 10 mm. b Schematic diagram showed circLRFN5 inhibits GSCs viability, proliferation and tumorigenesis and promotes ferroptosis via PRRX2 degradation and GCH1 downregulation (http://www.figdraw.com). d Kaplan–Meier survival curves showed the survival times of vector control group, circLRFN5 overexpressed group and combined with PRRX2 or GCH1 overexpression group. e Representative immunohistochemical images showed the staining and expression of PRRX2, GCH1 and Ki-67 of the vector control group, circLRFN5 overexpressed group and combined with PRRX2 or GCH1 overexpression group. Scale bar = 50 μm. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001