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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma

Fig. 3

Elevated B3GALT4 inhibits NB progression in vitro and in vivo. CCK-8 (A) and colony formation assays (B, C) were performed to determine the proliferation of 9464D and 975A2 cells transfected with B3GALT3 overexpression and knockdown lentivirus. Transwell assays were performed to analyze the invasion and migration abilities of 9464D (D) and 975A2 (E) cells. F Approximately 2 × 1069464D cells infected with vector or overexpressed B3GALT4 plasmids were injected subcutaneously into the back of C57BL/6 mice (n = 5 mice per group). G After 27 days, the mice were sacrificed, and the tumors were harvested. H The tumor volumes were measured every 3 days and shown in the line chart (Left). The final weights of tumors were calculated (Right). I IHC staining for Ki-67 in the indicated tumors. Scale bar: 100 μm

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