Fig. 4

B3GALT4 modulates CD8+ T lymphocyte migration and tumor infiltration via CXCL9 and CXCL10. A GSEA analysis indicated that B3GALT4 overexpression was enriched in T cell chemotaxis and chemokine-mediated signaling pathways based on data from the GEO database (GSE49710). B Integrated molecular analysis of mRNA expression of genes from the GSE49710 dataset was performed based on the B3GALT4 mRNA expression level. C Box plot demonstrating CD8A mRNA expression level in NB tissues (stages 1 + 2 + 4 s versus stages 3 + 4) from the GSE49710 dataset. D Survival analysis showed CD8A expression levels were significantly related to EFS and OS of NB patients in the GSE49710 dataset. E Correlation analysis between B3GALT4, CD8A, and chemokines (CXCL9 and CXCL10) mRNA expression levels in the GSE49710 database. F Schematic diagram of in vivo chemotaxis assay of CD8+ T cell migration. G Transwell assay analysis of CD8+ T cell migration ability in the supernatants of tumor cells with different treatments. H The percentages of CD8+ T cells in tumors from subcutaneous xenograft mouse models were assessed by FACS. Bar graphs showed the mean ± SD of three independent experiments. I IHC was done to evaluate CD8, CXCL9, and CXCL10 expression in NB tissues derived from tumor xenografts in mice. J IHC staining results of the B3GALT4, CD8A, CXCL9, and CXCL10 expression in tumor samples from NB patients with B3GALT4 high (n = 13) or low expression (n = 12)