Fig. 5

Modulatory role of ganglioside GD2 on lipid rafts. A Immunofluorescence colocalization of GD2 and caveolin-1 in 9464D and 975A2 cells with silenced or overexpression of B3GALT4. Scale bar: 50 μm. B IHC was used to evaluate B3GALT4, GD2, and caveolin-1 expression in NB tissues derived from tumor xenografts in mice, n = 5. Scale bar: 100 μm. C Western blot was conducted to analyze the expression of caveolin-1 in lipid rafts of 9464D and 975A2 cells with treatment with ganglioside GD2 or GD2 mAb