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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma

Fig. 6

The c-Met/AKT/mTOR/IRF-1 pathway is essential for synthesizing CXCL9 and CXCL10 regulated by B3GALT4. A Western blot analysis of the protein level of c-Met and the phosphorylation of c-Met in lipid rafts of 9464D and 975A2 cells with treatment with hepatocyte growth factor (HGF) (40 ng/ml, 24 h). B GSEA analysis showed that B3GALT4 overexpression was enriched in PI3K/AKT/mTOR and interferon regulatory factor-1 (IFN-γ) pathways based on data from the GEO database (GSE49710). (C) Correlation analysis of CXCL9, CXCL10, and IRF-1 expression levels in human NB specimens from the GSE49710 database. D, E Western blot analysis of the protein level of mTOR, p-mTOR, AKT, p-AKT, IRF-1, CXCL9, and CXCL10 in 9464D and 975A2 cells. F Enzyme-linked immunosorbent assay (ELISA) analysis of CXCL9 and CXCL10 in the 9464D and 975A2 cells supernatants

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