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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: ARPC1B promotes mesenchymal phenotype maintenance and radiotherapy resistance by blocking TRIM21-mediated degradation of IFI16 and HuR in glioma stem cells

Fig. 5

ARPC1B disrupted the TRIM21-mediated IFI16 and HuR ubiquitylation and degradation. A, B Co-IP assays were performed with an anti-HA antibody on cell lysates from HEK293 cells transfected with Flag-ARPC1B alone or together with the indicated HA-IFI16 (A) or HA-HuR (B) constructs. The upper panel is a schematic representation of wild-type sequences and the indicated deletion mutants. C Western blot analysis of co-IPs in lysates of HEK293 cells transfected with the indicated Flag-ARPC1B constructs together with HA-IFI16 (left panel) or HA-HuR (right panel). The upper panel is a schematic representation of wild-type ARPC1B and the two indicated mutants. D, E Co-IP analysis revealing the impact of ARPC1B knockdown (D) or ARPC1B overexpression (E) on the interaction between TRIM21 and IFI16 or TRIM21 and HuR

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