Fig. 6

Overexpression of IFI16 or HuR reactivated the STAT3 and NF-κB signaling pathways in sh-ARPC1B GSCs. A Western blotting analysis of protein expression of ARPC1B, IFI16, HuR, P65, p-P65, STAT3, p-STAT3 and CD44 in GSC 20 cells treated with the indicated interventions. B The quantification of tumor sphere formation of GSC 267 and GSC 20 cells treated with the indicated interventions. C Limiting dilution assays of GSC 267 and GSC 20 cells treated with the indicated interventions. D Quantification of the apoptosis rates of GSC 267 and GSC 20 cells treated with the indicated interventions. E Quantification of DNA damage evaluated by the comet assay in GSC 267 and GSC 20 cells treated with the indicated interventions