Fig. 2

UBR7 inhibits HCC proliferation and tumorigenesis. A The clone formation sphere experiment analysed Huh-7 and HepG2 cells overexpressing or knocking out UBR7. B The overexpression or knockout of UBR7 cells constructs xenogeneic tumours subcutaneously in nude mice (left). Tumour volumes (mm³) (middle) and the weight (right) of mouse xenografts implanted with HepG2 cells. C The liver morphology of UBR7^WT (WT) and Alb-Cre;UBR7^fl/fl mice. D Survival analysis of UBR7^WT (n = 24) and Alb-Cre;UBR7^fl/fl mice (n = 19). E Representative H&E staining images for sections of livers in UBR7^WT and Alb-Cre;UBR7^fl/fl mice. F Quantification of tumour area (%), number, maximal size (mm) (left), and Ki67 positive cells (%) (right) in UBR7^WT and Alb-Cre;UBR7^fl/fl mice. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.01. The scale bar in A and E was 100 μm