Fig. 3
From: UBR7 inhibits HCC tumorigenesis by targeting Keap1/Nrf2/Bach1/HK2 and glycolysis
UBR7 inhibits glycolysis by negatively regulating HK2. A Left: heatmap summarising genes differentially expressed in the Alb-Cre;UBR7fl/fl livers compared to UBR7WT (WT) livers via RNA-sequencing (RNA-seq) analysis. Right: volcano plots of the differentially expressed genes with 104 up-regulated genes and 369 down-regulated genes. B Left: Gene Ontology (GO) analysis showed different genes in (A). Right: Gene Set Enrichment Analysis (GSEA) output images of two chosen pathways displaying a correlation of differentially regulated genes in Alb-Cre;UBR7fl/fl liver with the “Metabolism core gene” set and “HK2-based core gene” set. C Immunohistochemical and Western blot analysis of the expression level of HK2 in Alb-Cre;UBR7fl/fl and WT liver. D WT and Alb-Cre;UBR7fl/fl mouse primary liver cells were analysed for glycolysis efficiency, the oxygen consumption rate (OCR), glucose uptake and lactate secretion levels. E, F Glycolysis efficiency, the oxygen consumption rate (OCR), glucose uptake and lactate secretion levels were detected in UBR7 overexpressing BEL-7402 or UBR7 knockout HepG2 cells. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. The scale bar in (C) is 100 μm