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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: UBR7 inhibits HCC tumorigenesis by targeting Keap1/Nrf2/Bach1/HK2 and glycolysis

Fig. 5

UBR7 promotes Keap1 expression levels by ubiquitinating H2BK120ub on Keap1. A Heatmap summarising chromatin immunoprecipitation (ChIP)-seq data for H2BK120ub, comparing liver cells from WT and Alb-Cre;UBR7fl/fl mice. B The Venn diagram shows that the 240 genes combined with H2BK120ub in the ChIP-seq analysis and the genes regulated in the RNA-seq analysis overlap. C Diagram of metabolic process genes. x-axis, gene expression from RNA-seq data; y-axis, H2BK120UB binding level determined in ChIP-seq data. D Keap1 gene peak in liver cells from WT and Alb-Cre;UBR7fl/fl mice. E The average gene density map of H2BK120Ub binding sites in Control (SCR) and UBR7-shRNA-expressing Huh-7 cells. F Left: Bar plot for quantitative PCR (qPCR) enrichment of UBR7 chromatin immunoprecipitation (ChIP) in Huh-7 cells for Keap1. GAPDH was used as a negative control. Right: Bar graph of qPCR enrichment of H2BK120Ub ChIP in Huh-7 cells expressing SCR or UBR7-shRNA. GAPDH was used as a negative control. G, H) Keap1 protein or mRNA expression levels in UBR7 KO primary hepatocytes and HepG2 cells, and Huh-7 cells overexpressing UBR7. I Fluorescein reporter gene analysis UBR7 bound to H2B on Keap1 in Alb-Cre;Ubr7fl/fl primary hepatocytes and HepG2 cells, and Huh-7 cells overexpressing UBR7. J The expression level of Keap1 in WT and Alb-Cre;UBR7fl/fl mice liver tissue was detected by immunohistochemistry. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. The scale bar in J was 100 μm

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