Fig. 3

Combined effects of JQ1/PAN on global gene expression. Using RNA-seq, gene expression studies were performed in HD-MB03 cells 24 h after treatment with DMSO (vehicle control), JQ1 (0.5 µM), PAN (10 nM) or the combination of JQ1 and PAN. A Venn-diagrams showing number of genes upregulated or downregulated by JQ1 and PAN alone or combined, compared to DMSO. B GSE analysis for the modulation of MYC/HDAC target gene sets by the combination of JQ1 and PAN, compared to DMSO. FDR, false discovery rate; ES, enrichment score. C Heatmap showing the top 50 genes most significantly downregulated by JQ1-PAN combination treatment