Fig. 4

Combined effects of BET and HDAC inhibitors on SYK and MSI1 expression. A Quantitative-PCR and B western blot analyses for the mRNA and protein expression of BRD4, SYK, MSI1 and MYC in MB HD-MB03 and D-283 cell lines treated for 24 h with 0.5 µM JQ1, 10 nM PAN alone, or both combined. GAPDH and Cyclophillin-B (cyclo)/ Vinculin were used as the loading controls in q-PCR and western blot analyses, respectively. Results are the representative of three independent replicates. Plotted values and error bars represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (Student-t-test). C Western blot analysis for the expression of BRD4, SYK, MSI1 and MYC proteins in HD-MB03 cells transfected with scrambled (control-siRNAs) and BRD4-siRNAs (50 nM) for 48 h