Fig. 4

Co-knockdown of AQP5 and LGR5 significantly attenuates the malignant biological function of GC-CSCs. (a-e) AQP5 and/or LGR5 were knocked down in AGS cells, and the cells were analyzed to assess sphere formation in serum-free culture (a); cell migration (b); cells were diluted and subcutaneously injected into severely immunodeficient mice (c). Tumors were examined over a 28-day period (n = 4 for each group). The tumor volume (d) and tumor weight (e) were monitored in the indicated groups and at the indicated time points