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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: OTUD4-mediated GSDME deubiquitination enhances radiosensitivity in nasopharyngeal carcinoma by inducing pyroptosis

Fig. 3

GSDME-dependent pyroptosis sensitizes NPC to radiotherapy in vivo. A Schematic view of the radiotherapy xenograft model. Cells stably expressing luciferase, SUNE1-Vector, SUNE1-GSDME, HONE1-Scramble, or HONE1-shGSDME, were subcutaneously injected into the nude mice (n = 28 mice per group). When tumor volumes reached 50–100 mm.3, mice bearing each type of tumor were randomized into a control group and a radiotherapy group (n = 14 in each subgroup). The mice in the radiotherapy group were irradiated with 12 Gy (2 Gy/day for 6 days). B Tumor size was monitored every 4 days (n = 5). C Representative bioluminescence images of tumors were taken on day 42. D Statistical analysis of photon flux (n = 5). E Images of resected tumors. F Tumor weight on day 42 (n = 5). G Serum LDH concentrations were determined pre-radiotherapy and following the third and sixth radiotherapy treatments (n = 3). H Representative images of HE (top) and IHC (bottom) staining of the tumor sections are shown. The dashed lines circumscribe the areas of tumor necrosis. I Western blotting showed the level of GSDME-N in resected tumor tissues. GAPDH was used to normalize the amount of protein loaded. Each bar represents the mean ± SD of three independent experiments. ns, No significant difference. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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